{"id":215,"date":"2025-05-10T08:09:03","date_gmt":"2025-05-10T08:09:03","guid":{"rendered":"https:\/\/schobot.net\/en\/?p=215"},"modified":"2025-05-10T08:18:27","modified_gmt":"2025-05-10T08:18:27","slug":"sustainable-quick-quantitative-screening-for-the-physiology-of-chitinases-production-by-bacillus-thuriengiensis-gallariae-and-detection-of-aedes-aegypti-biopesticide-activity","status":"publish","type":"post","link":"https:\/\/schobot.net\/en\/2025\/05\/10\/sustainable-quick-quantitative-screening-for-the-physiology-of-chitinases-production-by-bacillus-thuriengiensis-gallariae-and-detection-of-aedes-aegypti-biopesticide-activity\/","title":{"rendered":"Sustainable quick quantitative screening for the physiology of chitinases production by Bacillus thuriengiensis gallariae and detection of Aedes aegypti biopesticide activity"},"content":{"rendered":"\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

Sustainable quick quantitative screening for the physiology of chitinases production by Bacillus thuriengiensis gallariae and detection of Aedes aegypti biopesticide activity<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t

Author(s):<\/strong> Roshdy, Amira M.a, Abou-Baker, Nesreen H.b, Saber, Mohamedc, Hamed, Shimaa R.d, Sharaf, Omaima A.c, El-Bendary, Magda A.a, Amer, Mai N.e<\/p>

Keywords:<\/strong>\u00a0biocontrol, chitinases assay, colloidal chitin and Ostwald viscometer<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t

Abstract<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t

Background<\/strong><\/p>

Chitin is considered the world\u2019s first and most abundant amino-carbon substrate.<\/p>

Objective<\/strong><\/p>

The proposed method aims to speed up the detection and measuring of microbial chitinase production.<\/p>

Materials and methods<\/strong><\/p>

Solid-state fermentation, plates, viscosity, and spectrophotometric methods were used to detect microbial chitinase activity. Analysis of variance was used for statistical analysis.<\/p>

Results and conclusion<\/strong><\/p>

The trailed Bacillus thuriengiensis gallariae strain was differentiated from other tested strains of Bacillus thuriengiensis in terms of mosquito resistance, besides being superior to other studied Bacillus sphaericus. As anticipated, results from the clear zone plates method were not clear compared with the viscosity method where the strain exhibited the best. Worthy, the same result was reached in the color methods. The superlative incubation period for the physiological properties of chitinase production from Bacillus thuriengiensis gallariae was attained after 5 days. Results indicated that the best size of chitin particles was 1\u2009mm, the best percentage of humidity of the growth was 67%, the best inoculum volume was 10\u2009ml, and the best carbon source for the production of crude chitinases was glucose. The optimal concentration for the production of chitinases was 20% of the weight of the growth medium, yet 5% of the weight of the growth medium was the optimal percentage of glucose as a carbon source for the production of other protein metabolites. The proposed colorimetric method seemed to be an effective and rapid method to survey the aptitude of huge quantities of microorganisms to produce chitinases.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t

References<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\t

1.Kidibule PE, Santos-Moriano P, Ploub FJ, Fern\u00e1ndez-Lobatoa M. Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity. Biotechnol Rep 2020; 27:e00500. Cited Here | CrossRef | Google Scholar<\/p>

2. Ekundayo FO, Folorunsho AE, Ibisanmi TA, Olabanji OB. Antifungal activity of chitinase produced by Streptomyces species isolated from grass and soils in Futa Area, Akure. Bull Nati Res Cent 2022: 46\u201395. Cited Here | Google Scholar<\/p>

3. Fleuri LF, Kawaguti HY, Sato HH. Production, purification and application of extracellular chitinase from Cellulosimicrobiumcellulans191. Braz J Microbiol 2009; 40:623\u2013630. Cited Here | Google Scholar<\/p>

4. Kuddus M. Potenial applications of microbial chitinase: Recent Development. Biochem. Cell. Arch. 2014; 14:1\u20137. Cited Here | PubMed | Google Scholar<\/p>

5. Swiontek Brzezinska M, Jankiewicz U, Burkowska A, Walczak M. Chitinolytic microorganisms and their possible application in environmental protection. CurrMicrobiol 2014; 68:71\u201381. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

6. Yan Q, Fong SS. Bacterial chitinase: nature and perspectives for sustainable bioproduction. Bioresources and Bioprocessing 2015; 2:3. Cited Here | Google Scholar<\/p>

7. Singh M, Meena SC, Kumarjain R. Application of chitinase for sustainable agriculture. Adv Life Sci 2016; 5:11131\u201311138. Cited Here | Google Scholar<\/p>

8. Pramesti E, Puspita ID. Optimization of colloidal chitin and inoculum concentration in chitinase production by streptomyces sp. PB2 using response surface methodology. E3S Web of Conferences 2020; 147:03011. Cited Here | Google Scholar<\/p>

9. Taokaew S, Kriangkrai W. Chitinase-assisted bioconversion of chitinous waste for development of value-added chito-oligosaccharides products. Biology 2023; 12:87. Cited Here | Google Scholar<\/p>

10. Sharma A, Arya SK, Singh J, Kapoor B, Bhatti BS, Suttee A, Singh G. Prospects of chitinase in sustainable farming and modern biotechnology: an update on recent progress and challenges. Biotechnol Genet Eng Rev 2023; 1:1\u201331. Cited Here | CrossRef | Google Scholar<\/p>

11. Smith JR, Grula EA. Chitinase is an inducible Enzyme in Beauveriabassiana. J Invertebr Pathol 1983; 42:319\u2013326. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

12. Jeuniaux C. Chitinases. Chapter 111, in: Methods in Enzymology. 1966;8: 644\u2013650. Cited Here | Google Scholar<\/p>

13. Roopavathi AS, Vigneshwari R, Jayapradha R. Chitinase: production and applications. J Chem Pharm Res 2015; 7:924\u2013931. Cited Here | Google Scholar<\/p>

14. Mart\u00ednez-Zavala SA, Barboza-P\u00e9rez UE, Hern\u00e1ndez-Guzm\u00e1n G. Chitinases of Bacillus thuringiensis: phylogeny, modular structure, and applied potentials. Front Microbiol 2020; 14:3032. Cited Here | Google Scholar<\/p>

15. Jeuniaux C. Recherchessur les chitinase. I. Dosage nephelometrique et production de chitinase par des Streptomycetes. Arch Intern PhysiolBiochim 1958; 66:408\u2013427. Cited Here | Google Scholar<\/p>

16. Onen H, Luzala MM, Kigozi S, Sikumbili RM, Muanga CJK, Zola EN, et al. Mosquito-Borne Diseases and Their Control Strategies: An Overview Focused on Green Synthesized Plant-Based Metallic Nanoparticles. Insects 2023; 14:221. Cited Here | Google Scholar<\/p>

17. Baranek J, Jakubowska M, Gaba\u0142a E. Insecticidal activity of Bacillus thuringiensis towards Agrotisexclamationis larvae-A widespread and underestimated pest of the Palearctic zone. PLoS ONE 2023; 18:e0283077. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

18. Berini F, Casartelli M, Montali A, Reguzzoni M, Tettamanti G, Marinelli F. Metagenome-aourced microbial chitinases as potential insecticide proteins. Front. Microbiol 2019; 10:1358. Cited Here | Google Scholar<\/p>

19. Roshdy AM, Shata HM, Ali SM, Youssef AM, Foda MS, Kahil T. Commercial extraction of applicable alkaline protease and chitinase by Bacillus thuringiensis dendrolimus IP 4A\/4B. J Mod AgricBiotechnol 2022; 1:21. Cited Here | Google Scholar 20. Boller T, Gehri A, Mauch F. V\u04e7geli U. Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. Planta 1983; 157:22\u201331. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

21. Mehrotra AK, Monnery WD, Svrcek WY. A review of practical calculation methods for the viscosity of liquid hydrocarbons and their mixtures. Fluid Phase Equilibria 1996; 117:344\u2013355. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

22. Stevens RA, Levin RE. Viscometric Assay of Bacterial Alginase. Appl Environ Microbiol 1976; 31:896\u2013899. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

23. Jha SC, Modi HA. Comparative Analysis of Chitinase Activity by Four Different Assay from Soil Born Actinomycetes. In 4th International Conference on Multidisciplinary Research & Practice, Ahmedabad, India, 2017. 185\u2013190 Cited Here | Google Scholar<\/p>

24. Abdel-Salam MS, Ameen Hoda H. Soliman, Gaziea M, Elkelany U S, Asar Amira M. Improving the nematicidal potential of Bacillus amyloliquefaciens and Lysinibacillus sphaericus against the root-knot nematode Meloidogyne incognita using protoplast fusion technique. Egyptian. J Biol Pest Cont 2018; 28:31. Cited Here | Google Scholar<\/p>

25. Afify A, Aboul-Soud M, Foda M, Sadik M, Kahil T, Amira R, et al. Production of alkaline protease and larvicidalbiopesticides by an Egyptian Bacillus sphaericus isolate. Afr J Biotechnol 2009; 8:3864\u20133873. Cited Here | PubMed | Google Scholar<\/p>

26. Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research. New York, USA: Jon Willey and Sons Inc; 1984:2. Cited Here<\/p>

27. Karimian Z, Sabbaghian Z, Salehi A, Sedghpour BS. Obstacles to undertaking research and their effect on research output: a survey of faculty members\u2019 views at Shiraz University of Medical Sciences. EMHJ 2012; 18:1143\u20131150. Cited Here | Google Scholar<\/p>

28. Ferrari AR, Gaber Y, Fraaije MW. A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection. Biotechnolr Biofuels 2014; 7:37. Cited Here | Google Scholar<\/p>

29. Senthilkumar M, Amaresan N, Sankaranarayanan A. Plant-Microbe Interactions Laboratory Techniques, Springer Protocols Handbooks 2021 https:\/\/doi.org\/10.1007\/978-1-0716-1080-0_58. Science Business Media, LLC, part of Springer Nature. Cited Here | Google Scholar<\/p>

30. Shen C, Chen Y, Yang C, Chen J, Liu C. Colloid chitin azure is a dispersible, low-cost substrate for chitinase measurements in a sensitive, fast, reproducible assay. J Biomol Screen 2010; 15:213\u20137. Cited Here | PubMed | Google Scholar<\/p>

31. Klassen J. MSU: CHMY 362 Eelements of Physical Chemistry. Montana State University in Bozeman 2023. Cited Here | Google Scholar<\/p>

32. Wright RJ, Gibson MI, Christie-Oleza JA. Artificial selection of microbial communities to enhance degradation of recalcitrant polymers 2018. Cited Here | Google Scholar<\/p>

33. Baumann P, Clark MA, Baumann L, Broadwell AH. Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins. Microbiol Rev 1991; 55:425\u201336. Cited Here | PubMed | CrossRef | Google Scholar<\/p>

34. Kumar AS, Moorthi M, Ramakrishnan N, Dhanapakiam P, Ramasamy VK, Rajeswari AR. Efficacy of Bacillus thuringiensis var. Kurstakiin the control of two mosquito species (Anopheles stephensi and Culexquinquefasciatus). J Environ Biol 2009; 30:433\u20135. Cited Here | PubMed | Google Scholar<\/p>

35. Abu El-Ghiet UM, Moustafa SA, Ayashi MM, El-Sakhawy MA, Ateya AAE, Waggiallah HA. Characterization of Bacillus thuringiensis isolated from soils in the Jazan region of Saudi Arabia, and their efficacy against Spodoptera littoralis and Aedes aegypti larvae. Saudi J Biol Sci 2023; 30:103721. Cited Here | Google Scholar<\/p>

36. Ayilara MS, Adeleke BS, Akinola SA, Fayose CA, Adeyemi UT, Gbadegesin LA, et al. Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Front. Microbiol 2023; 16:1040901. Cited Here | Google Scholar 37. Foda MS, Ali SM, Youssef AM, Kahil T, Hoda MS, Roshdy AM. Production Physiology of Alkaline Protease by Bacillus thuringiensisspp. under Solid-State Fermentation Conditions. J Appl Sci Res 2013; 9:1975\u20131984.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Sustainable quick quantitative screening for the physiology of chitinases production by Bacillus thuriengiensis gallariae and detection of Aedes aegypti biopesticide activity Author(s): Roshdy, Amira M.a, Abou-Baker, Nesreen H.b, Saber, Mohamedc, Hamed, Shimaa R.d, Sharaf, Omaima A.c, El-Bendary, Magda A.a, Amer, Mai N.e Keywords:\u00a0biocontrol, chitinases assay, colloidal chitin and Ostwald viscometer Abstract Background Chitin is considered […]<\/p>\n","protected":false},"author":28,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[29,30,31],"class_list":["post-215","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-biocontrol","tag-chitinases-assay","tag-colloidal-chitin-and-ostwald-viscometer"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/posts\/215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/users\/28"}],"replies":[{"embeddable":true,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/comments?post=215"}],"version-history":[{"count":7,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/posts\/215\/revisions"}],"predecessor-version":[{"id":247,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/posts\/215\/revisions\/247"}],"wp:attachment":[{"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/media?parent=215"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/categories?post=215"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/schobot.net\/en\/wp-json\/wp\/v2\/tags?post=215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}